MotrpacWorkshops

MoTrPAC Workshops

This repository provides interactive R notebooks and tutorials to guide researchers through the analysis of comprehensive multi-omics datasets generated by the Molecular Transducers of Physical Activity Consortium (MoTrPAC). These workshops demonstrate how to leverage MoTrPAC R packages to explore exercise-induced molecular changes across multiple tissues and omics platforms.

Available Workshops

1. Introduction to MoTrPAC Endurance Training Analysis

Notebook: rat-endurance-6m.Rmd (view html version)

This comprehensive tutorial introduces the MoTrPAC endurance exercise training dataset from young rats. You’ll learn to:

• Install and configure the MotrpacRatTraining6moData and MotrpacRatTraining6mo packages
• Navigate the multi-omics dataset spanning 19 tissues and 7 omics platforms (transcriptomics, proteomics, metabolomics, etc.)
• Retrieve differential analysis results across training time points (1w, 2w, 4w, 8w)
• Visualize temporal trajectories of molecular responses to endurance training
• Compare sex-specific differences in training adaptations
• Generate publication-ready plots and heatmaps

Ideal for: Researchers new to the MoTrPAC dataset who want to understand the overall data structure and available analysis functions.

2. Custom Gene List Analysis Across Multi-Omics Layers

Notebook: rat-training-6m-custom-gene-list.Rmd (view html version)

This interactive workflow enables hypothesis-driven exploration of exercise responses for your genes of interest. You’ll learn to:

• Input custom gene lists and automatically map them across omics platforms
• Retrieve differential analysis results for your specific genes across all available tissues
• Generate interactive tables with searchable and sortable results
• Create beautiful trajectory plots showing gene expression changes over the training time course
• Build heatmaps with significance markers comparing female vs. male responses
• Identify tissue-specific and sex-specific exercise adaptations

Ideal for: Researchers with specific genes or pathways of interest who want to explore how they respond to endurance training across the entire MoTrPAC dataset.

Getting Started

All vignettes are available in the vignettes/ directory and can be rendered as HTML documents for easy viewing and interaction. The workshops use real data from the MoTrPAC study and demonstrate reproducible analysis workflows.

This site is open source. Improve this page.