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Median-MAD normalized protein acetylation (ACETYL) data used for visualization and differential analysis

Usage

ACETYL_HEART_NORM_DATA

ACETYL_LIVER_NORM_DATA

Format

A data frame with acetylsites in rows (feature_ID) and samples in columns (viallabel)

An object of class data.frame with 9750 rows and 57 columns.

Source

pass1b-06/analysis/proteomics-untargeted/prot-ac/normalized-data/*med-mad-normalized-logratio.txt

Details

Log2 TMT ratios to the common reference were used as quantitative values for all proteomics features (proteins, phosphosites, acetylsite, and ubiquitylsites). Proteomics features not fully quantified in at least two plexes within a tissue and non-rat contaminants were removed. Log2 TMT ratios were sample-normalized by median-centering and mean absolute deviation scaling. Plex batch effects were removed using linear models implemented by the limma::removeBatchEffect().