An object of class
Biobase::ExpressionSet
containing median-MAD-normalized log\(_2\)-transformed protein expression
data for differential analysis and WGCNA.
PROT_EXP
Object of class
Biobase::ExpressionSet
with 9964
features and 60 samples.
See FEATURE_TO_GENE
for
details of feature-to-gene mapping.
library(Biobase)
PROT_EXP # display summary
#> ExpressionSet (storageMode: lockedEnvironment)
#> assayData: 9964 features, 60 samples
#> element names: exprs
#> protocolData: none
#> phenoData
#> sampleNames: 90222017006 90258017006 ... 90237017006 (60 total)
#> varLabels: pid bid ... exp_group (7 total)
#> varMetadata: labelDescription
#> featureData
#> featureNames: AP_004893.1 AP_004894.1 ... YP_665639.1 (9964 total)
#> fvarLabels: feature_ID gene_symbol entrez_gene
#> fvarMetadata: labelDescription
#> experimentData: use 'experimentData(object)'
#> Annotation:
exprs(PROT_EXP)[1:10, 1:5] # assayData (first 10 rows and 5 columns)
#> 90222017006 90258017006 90441017006 90266017006 90421017006
#> AP_004893.1 0.06250 -0.00673 0.06530 -0.12847 0.42877
#> AP_004894.1 -0.35726 -0.00678 -0.00001 -0.09093 0.34666
#> AP_004895.1 -0.29374 0.16927 0.04853 -0.08151 0.34469
#> AP_004896.1 0.03139 -0.05546 0.06703 -0.09930 0.22900
#> AP_004898.1 -0.20785 0.18767 0.10063 -0.33669 0.15039
#> AP_004899.1 NA NA NA NA NA
#> AP_004901.1 NA NA NA NA NA
#> AP_004904.1 -0.12326 -0.35704 -0.24179 -0.01929 0.79072
#> NP_001000516.1 -0.42419 0.11770 0.41690 -0.09733 -0.35746
#> NP_001001505.1 -0.17732 -0.13575 -0.03853 0.15163 -0.19405
head(fData(PROT_EXP)) # featureData
#> feature_ID gene_symbol entrez_gene
#> AP_004893.1 AP_004893.1 Mt-nd2 26194
#> AP_004894.1 AP_004894.1 Mt-co1 26195
#> AP_004895.1 AP_004895.1 Mt-co2 26198
#> AP_004896.1 AP_004896.1 Mt-atp8 26196
#> AP_004898.1 AP_004898.1 Mt-co3 26204
#> AP_004899.1 AP_004899.1 Mt-nd3 26199
head(pData(PROT_EXP)) # phenoData
#> pid bid labelid viallabel sex timepoint exp_group
#> 90222017006 10025626 90222 4.457560e-313 90222017006 Male 8W M_8W
#> 90258017006 10045228 90258 4.459338e-313 90258017006 Female 8W F_8W
#> 90441017006 10502300 90441 4.468380e-313 90441017006 Male 2W M_2W
#> 90266017006 10046380 90266 4.459734e-313 90266017006 Female SED F_SED
#> 90421017006 10417702 90421 4.467392e-313 90421017006 Male 1W M_1W
#> 90410017006 10335064 90410 4.466848e-313 90410017006 Female 4W F_4W